Assessment of Changes in Morphology and Wound Closure Rate of Skin Tissue Defect Against the Background of Standard Therapy for Cold Injury and When Using Biodegradable Tissue-Engineered Products
https://doi.org/10.22448/AMJ.2026.1.51-59
EDN: VDBHCH
Abstract
Background. The search for modifications of biodegradable tissue-engineered products (BTEP) for the treatment of deep frostbite remains an urgent problem in the medicine of critical temperature exposures.
Objective. To study the morphology and closure rate of deep skin frostbite wound defect in laboratory animals using standard therapy and its combination with BTEP implantation.
Materials and methods. The study was performed on 70 male Wistar rats. Rats of the control and experimental groups were modeled with deep contact frostbite of III degree of the back skin, after which the control group received standard frostbite therapy, the experimental group additionally received BTEP implantation. Animals were sacrificed on days 3, 7 and 14 of the experiment. The wound defect closure rate and morphometric parameters in skin preparations were determined.
Results. On day 3, plethoric vessels with persistent spasm were determined in the control and experimental groups. On day 7, in the experimental group, active ingrowth of dermal fibers into the BTEP implantation zone was observed, the wound epithelialization rate was higher (p=0.037). On day 14, a branched microvasculature network without signs of congestive insufficiency was formed in the experimental group.
Conclusion. The use of BTEP accelerates dermal regeneration, ensuring the formation of full-fledged connective tissue.
Keywords
Supporting agencies: The study was not sponsored.
About the Authors
S. V. Barannikov
Amur State Medical Academy
Russian Federation
Russian Federation
Sergey V. Barannikov
Blagoveshchensk
T. S. Churikova
Amur State Medical Academy; Far Eastern Scientific Center of Physiology and Pathology of Respiration
Russian Federation
Russian Federation
Tatiana S. Churikova
Blagoveshchensk
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For citations:
Barannikov S.V., Churikova T.S. Assessment of Changes in Morphology and Wound Closure Rate of Skin Tissue Defect Against the Background of Standard Therapy for Cold Injury and When Using Biodegradable Tissue-Engineered Products. Amur Medical Journal. 2026;14(1):51-59. (In Russ.) https://doi.org/10.22448/AMJ.2026.1.51-59. EDN: VDBHCH
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